Christopher Daniel Barnes

Peter Parker / Spider-Man (voice)

Sara Ballantine

Mary Jane Watson (voice)

Ed Asner

J. Jonah Jameson (voice)

Roscoe Lee Browne

Wilson Fisk / Kingpin (voice)

Jennifer Hale

Felicia Hardy (voice)

Linda Gary

Aunt May (voice)

Julie Bennett

Aunt May (voice)

Wanda De Jesus

Doctor Sylvia Lopez (voice)

Mark Hamill

Jason Phillips / Hobgoblin (voice)

Dorian Harewood

Tombstone (voice)

Gary Imhoff

Harry Osborn (voice)

Nick Jameson

Richard Fisk (voice)

Oliver Muirhead

Doctor Jonathan Ohn / The Spot (voice)

Neil Ross

Norman Osborn / Green Goblin (voice)

Rodney Saulsberry

Joe 'Robbie' Robertson (voice)

David Warner

Landon (voice)

Carl Ballantine

Lenny Luntz (voice)

Majel Barrett

Anna Watson (voice)

Bob Bergen

Ned Leeds (voice)

Joseph Campanella

Doctor Curt Connors (voice)

Nell Carter

Glory Grant (voice)

Frankie Crocker

Jimmie Dugan (voice)

Liz Georges

Debra Whitman (voice)

Robert Hays

Stark (voice)

Patrick Labyorteaux

Flash Thompson (voice)

Joan Lee

Madame Web (voice)

Iona Morris

Martha Robertson (voice)

Lois Nettleton

Nora (voice)

Alfonso Ribeiro

Randy Robertson (voice)

Stanley Ralph Ross

Joey Nails (voice)

Marla Rubinoff

Liz Allen (voice)