Bob Hoskins

Eddie Valiant

Christopher Lloyd

Judge Doom

Joanna Cassidy

Dolores

Charles Fleischer

Roger Rabbit / Benny the Cab / Greasy / Psycho (voice)

Kathleen Turner

Jessica Rabbit (voice) (uncredited)

Stubby Kaye

Marvin Acme

Alan Tilvern

R.K. Maroon

Richard LeParmentier

Lt. Santino

Lou Hirsch

Baby Herman (voice)

Betsy Brantley

Jessica's Performance Model

Joel Silver

Raoul

Paul Springer

Augie

Richard Ridings

Angelo

Edwin Craig

Arthritic Cowboy

Lindsay Holiday

Soldier

Mike Edmonds

Stretch

Morgan Deare

Editor / Gorilla (voice)

Danny Capri

Kid #1

Christopher Hollosy

Kid #2

Jean-Paul Sipla

Kid #3

Laura Frances

Blonde Starlet

Joel Cutrara

Forensic #1

Billy J. Mitchell

Forensic #2

Eric B. Sindon

Mailman

Ed Herlihy

Newscaster

James O'Connell

Conductor

Eugene Gutierrez

Teddy Valiant

April Winchell

Mrs. Herman (voice)

Mae Questel

Betty Boop (voice)

Mel Blanc

Daffy Duck / Tweety / Bugs Bunny / Sylvester / Porky Pig (voice)

Tony Anselmo

Donald Duck (voice)

Mary T. Radford

Hippo (voice)

Joe Alaskey

Yosemite Sam / Foghorn Leghorn (voice)

David L. Lander

Smart Ass (voice)

Fred Newman

Stupid (voice)

June Foray

Wheezy / Lena Hyena (voice)

Wayne Allwine

Mickey Mouse (voice)

Russi Taylor

Minnie Mouse / Birds (voice)

Les Perkins

Mr. Toad (voice)

Richard Williams

Droopy (voice)