Christopher Daniel Barnes

Peter Parker / Spider-Man (voice)

Sara Ballantine

Mary Jane Watson (voice)

Efrem Zimbalist Jr.

Doctor Octopus (voice)

Neil Ross

Norman Osborn / Green Goblin (voice)

Billy 'Pop' Atmore

Robert Farrell / Rocket Racer (voice)

Ed Asner

J. Jonah Jameson (voice)

Gary Imhoff

Harry Osborn (voice)

Joan Lee

Madame Web (voice)

Dimitra Arliss

Anastasia Hardy (voice)

Linda Gary

Aunt May (voice)

Jennifer Hale

Felicia Hardy (voice)

Michael Des Barres

Jackson Weele (voice)

Majel Barrett

Anna Watson (voice)

Rodney Saulsberry

Joe 'Robbie' Robertson (voice)

Marla Rubinoff

Liz Allen (voice)

Patrick Labyorteaux

Flash Thompson (voice)

Stephanie Eustase

Maria Taina Elizando (voice)

Michael Rye

Doctor Farley Stillwell (voice)

Roscoe Lee Browne

Wilson Fisk / Kingpin (voice)

Anne-Marie Johnson

Mousie (voice)

Scott Cleverdon

Doctor Michael Pingree (voice)

Philip Abbott

Wardell Stromm (voice)

Dawnn Lewis

Detective Terri Lee (voice)

Telma Hopkins

Mrs. Farrell (voice)

Mark Hamill

Jason Phillips (voice)